General#

Cell Line

hPSCreg Name BCRTi005-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 4th October 2018
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Provider

Generator Berlin-Brandenburg Center for Regenerative Therapies (BCRT)
Owner Berlin-Brandenburg Center for Regenerative Therapies (BCRT)
Distributors
Derivation country Germany

External Databases

Cellosaurus CVCL_9S06

General Information

Publications
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex female
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Donor Relations

Other cell lines of this donor

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
kidney epithelial cell
An epithelial cell of the kidney.
Source cell origin
urine
Excretion that is the output of a kidney
Age of donor (at collection) 25-29
Passage number reprogrammed 5

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
goosecoid
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Brachyuri
Yes
MIXL1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
NEUROD1
Yes
OTX2
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Karyotyping method: KaryoLite™ BoBs™ (Perkin Elmer)

Other Genotyping (Cell Line)