Generation of a miR-26b stem-loop knockout human iPSC line, MCRIi019-A-1, using CRISPR/Cas9 editing


miR-26b has been implicated in a wide range of human diseases, including cancer, diabetes, heart disease, Alzheimer's disease and osteoarthritis. To provide a tool to explore the importance of miR-26b in this broad context, we have generated and characterized a homozygous miR-26b stem-loop knockout human iPSC line. This gene-edited line exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. This iPSC line will be valuable for studies investigating disease mechanisms and testing therapeutic strategies in vitro. Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Kung LHW, Sampurno L, Little CB, Lamandé SR, Bateman JF
Journal Stem cell research
Publication Date 2020 Dec 10;50:102118
PubMed 33316599
DOI 10.1016/j.scr.2020.102118

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