IPS(IMR90)-4, WAi004-B

General#

Cell Line

hPSCreg Name WISCi004-B
Alternative name(s)
IPS(IMR90)-4, WAi004-B
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 29th May 2019
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Provider

Generator University of Wisconsin (WISC)
Derivation country United States

External Databases

Cellosaurus CVCL_C437
BioSamples SAMEA5840405

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) fetal

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA5840265

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell line name IMR-90
Other names
Derived from same source line (potentially other lot and donor, see below):
Source cell type
IMR-90
Human Fetal Lung Fibroblast cell line
Synonyms
  • IMR90
Age of donor (at collection) fetal

Reprogramming method

Vector type Integrating
Vector Virus (Lentivirus)
Genes
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones Geneticin Selection
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells Mouse Embryonic Fibroblast
Cellfinder Ont Id: EFO_0004040
Medium mTeSR™ 2
Supplements
zbFGF 100 ng/ml

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SOX2
Yes
POU5F1 (OCT-4)
Yes
LIN28
Yes
NANOG
Yes
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Transcriptome Characterisation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46X
Karyotyping method: G-Banding

Other Genotyping (Cell Line)