UNIPDi005-A
General
Cell Line |
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| hPSCreg name | UNIPDi005-A |
| Cite as: | UNIPDi005-A (RRID:CVCL_YS96) |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
WISCi004-A (IPS(IMR90)-1) WISCi004-B (IPS(IMR90)-4, WAi004-B) |
| Last update | 25th April 2022 |
| User feedback | |
Provider |
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| Generator |
University of Padova (UNIPD)
Contact:
University of Padova (UNIPD) |
| Owner | University of Padova (UNIPD) |
| Distributors | |
| Derivation country | Italy |
External Databases |
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| BioSamples | SAMEA6456097 |
| Cellosaurus | CVCL_YS96 |
| Wikidata | Q98134314 |
General Information |
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| Publications | |
| Projects | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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| Sex | female |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Disease associated phenotypes | no phenotypes |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA5840265 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
| Please provide contact information of the holder of the original Donor Information Sheet. | ATCC |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | No |
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| Is there other documentation provided to the donor for consenting purposes? | No |
| Confirm that consent was obtained by a qualified professional | Yes |
| Has the donor agreed to be re-contacted? | Unknown |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | No |
| Does consent permit unforeseen future research, without further consent? | Yes |
| Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent permit research by | |
| an academic institution? | Yes |
| a public organisation? | Yes |
| How may genetic information associated with the cell line be accessed? | No information |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | IRB for Human Subjects Research of the Institute for Medical Research, Candem NJ - USA |
| Approval number | Please see document provided by the ATCC. |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | No |
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | No vectors were used. |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | Yes |
| Constraints for use or distribution | According to the IMR-90 MTA from ATCC, the |
hIPSC Derivation
General |
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| Source cell line name |
IMR-90 Other names
Derived from same source line (potentially other lot and donor, see below):
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| Source cell type | |
| Source cell origin |
The organs of respiration that develop during the fetal stage of growth.
Synonyms
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| Source cell line vendor | ATCC |
Reprogramming method |
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| Vector type | None |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
mRNA
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| mRNA | |
Other |
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| Selection criteria for clones | Morphology of iPS colonies |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Gelatin | |||||||||||||||
| Feeder cells |
Mouse Embryonic Fibroblasts Cellfinder Ont Id: EFO_0004040 |
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| Passage method |
Enzymatically
Dispase
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| O2 Concentration | 5 % | |||||||||||||||
| CO2 Concentration | 5 % | |||||||||||||||
| Medium |
Other medium:
Base medium: N2B27
Main protein source: Albumine Serum concentration: 0 % Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
Transcriptome analysis by RNA sequencing confirmed full acquisition of naive pluripotency, given the high similarity with other embryo-derived naive pluripotent stem cells.
Methylome analysis by RRBS also indicated a global DNA hypomethylation, as also observed in other naive pluripotent stem cells.
Formation of Embryoid bodies, expressing markers of the 3 germ layers.
In vitro directed differentiation towards Endoderm, Mesoderm or Ectoderm.
In vitro differentiation towards neuronal or hepatic cells.
Transcriptome Characterisation
Differentiation Potency
In vitro spontaneous differentiation
In vitro directed differentiation
In vitro spontaneous differentiation
In vitro directed differentiation
| Marker | Expressed |
| T |
Yes |
| GATA4 |
Yes |
| KDR / FLK1 |
Yes |
In vitro spontaneous differentiation
In vitro directed differentiation
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX Normal
Passage number: 11
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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