General#

Cell Line

hPSCreg Name UNIPDi005-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 3rd December 2019
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Provider

Generator University of Padova (UNIPD)
Owner University of Padova (UNIPD)
Distributors
Derivation country Italy

External Databases

BioSamples SAMEA6456097

General Information

Publications
Projects
* Is the cell line readily obtainable for third parties?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA5840265

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: ATCC
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? No
If you do not hold the SIGNED Donor Consent Form, do you know who does? No
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Alternatives to consent are available? Yes
Alternatives to consent
Alternative consent approval number
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Unknown
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent permit unforeseen future research, without further consent? Yes
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
an academic institution? Yes
a public organisation? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? IRB for Human Subjects Research of the Institute for Medical Research, Candem NJ - USA
Approval number Please see document provided by the ATCC.
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No
Is there an MTA available for the cell line? No
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? No vectors were used.
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? Yes
Constraints for use or distribution According to the IMR-90 MTA from ATCC, the

hIPSC Derivation#

General

Source cell line name IMR-90
Other names
Derived from same source line (potentially other lot and donor, see below):
Source cell type
lung fibroblast cell line
Source cell origin
Fetal Lung
The development of the lungs after the eleventh week of gestation until the time of birth.
Synonyms
  • Fetal Lung
Source cell line vendor ATCC

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA
mRNA

Other

Selection criteria for clones Morphology of iPS colonies
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Gelatin
Feeder cells Mouse Embryonic Fibroblasts
Cellfinder Ont Id: EFO_0004040
Passage method Enzymatically
Dispase
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: N2B27
Main protein source: Albumine
Serum concentration: 0 %
Supplements
Human LIF 10 ng/ml
PD0325901 1 µM
CHIR99021 1 µM
Gö6983 2 µM
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
NANOG
Yes
SOX2
Yes
PRDM14
Yes
TFCP2L1
Yes
KLF4
Yes
KLF17
Yes
DPPA3
Yes
DPPA5
Yes
Transcriptome analysis by RNA sequencing confirmed full acquisition of naive pluripotency, given the high similarity with other embryo-derived naive pluripotent stem cells. Methylome analysis by RRBS also indicated a global DNA hypomethylation, as also observed in other naive pluripotent stem cells. Formation of Embryoid bodies, expressing markers of the 3 germ layers. In vitro directed differentiation towards Endoderm, Mesoderm or Ectoderm. In vitro differentiation towards neuronal or hepatic cells.
Transcriptome Characterisation
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
SOX17
Yes
FOXA2
Yes
GATA6
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
T
Yes
GATA4
Yes
KDR / FLK1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
SOX1
Yes
SOX2
Yes
NES
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX Normal
Karyotyping method: G-Banding

Other Genotyping (Cell Line)