MNZTASi004-A

MS_0003

General

Cell Line

hPSCreg name MNZTASi004-A
Cite as:
MNZTASi004-A (RRID:CVCL_C0K4)
Alternative name(s)
MS_0003
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
ESi121-A
(RRMS PBiPS12-Sv4F-6)
Donor diseases:
Relapsing-Remitting Multiple Sclerosis
ESi120-A
(RRMS PBiPS11-Sv4F-7)
Donor diseases:
Relapsing-Remitting Multiple Sclerosis
ESi130-A
(RRMS PBiPS14-Sv4F-7)
Donor diseases:
Relapsing-Remitting Multiple Sclerosis
ESi129-A
(RRMS PBiPS13-Sv4F-1)
Donor diseases:
Relapsing-Remitting Multiple Sclerosis
Last update 8th November 2023
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Provider

Generator Menzies Institute for Medical Research (MNZTAS)
Owner Menzies Institute for Medical Research (MNZTAS)
Distributors
Derivation country Australia

External Databases

Cellosaurus CVCL_C0K4
Wikidata Q112930123
BioSamples SAMEA112866622

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: research in neurodegenerative diseases or other human health conditions
Research use: allowed

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • MS RELAPSING REMITTING
  • Remitting-Relapsing Multiple Sclerosis
  • Multiple Sclerosis, Relapsing-Remitting
  • Multiple Sclerosis, Remitting-Relapsing
  • Remitting Relapsing Multiple Sclerosis
  • Multiple Sclerosis, Acute Relapsing
  • RRMS
  • Relapsing-remitting MS
  • Acute Relapsing Multiple Sclerosis
  • relapsing-remitting multiple sclerosis
  • Relapsing Remitting Multiple Sclerosis
  • Multiple Sclerosis, Relapsing Remitting
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

External Databases (Donor)

BioSamples SAMEA112866622

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: Menzies Institute for Medical Research
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes

Does consent permit research by

an academic institution? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? University of Tasmania Human Research Ethics Committee
Approval number H0016915
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • PBMC
Source cell origin
The portion of the upper extremity between the shoulder and the elbow. For clinical purposes this term is also used to refer to the whole superior limb.
Synonyms
  • ARM
  • Arm
  • Brachium
  • Upper Arm
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Dispase
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
OCT4
Yes
SOX2
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
AFP
Yes
Morphology
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
HAND1
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
PAX6
Yes

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes

Other Genotyping (Cell Line)