Generation of three human iPSC lines from PLAN (PLA2G6-associated neurodegeneration) patients
Summary
The human iPSC cell lines, PLANFiPS1-Sv4F-1 (RCPFi004-A), PLANFiPS2-Sv4F-1 (RCPFi005-A), PLANFiPS3-Sv4F-1 RCPFi006-A), derived from dermal fibroblast from three patients suffering PLAN (PLA2G6-associated neurodegeneration; MIM 256600) caused by mutations in the PLA2G6 gene, was generated by non-integrative reprogramming technology using OCT3/4, SOX2, CMYC and KLF4 reprogramming factors. The pluripotency was assessed by immunocytochemistry and RT-PCR. Differentiation capacity was verified in vitro. This iPSC line can be further differentiated toward affected cells to better understand molecular mechanisms of disease and pathophysiology. Copyright © 2021. Published by Elsevier B.V.
| Authors | Machuca C, Correa-Vela M, García-Navas D, Darling A, Villalón-García I, Sánchez-Alcázar JA, Pérez-Dueñas B, Erceg S, Espinós C |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2021 May;53:102338 |
| PubMed | 34087982 |
| DOI | 10.1016/j.scr.2021.102338 |