LVIP01-SD1-1, KR-4F
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General
Cell Line |
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| hPSCreg name | LVPEIi007-A |
| Cite as: | LVPEIi007-A (RRID:CVCL_D6MV) |
| Alternative name(s) |
LVIP01-SD1-1, KR-4F
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 18th September 2025 |
| Notes | This is an autosomal recessive retinal dystrophy (ARRD) patient-specific, induced pluripotent stem cell line, carrying a homozygous point mutation within exon 44 of ABCA4 leading to Arg2030Ter. This mutation (c.6088C>T) results in premature truncation of the protein lacking C-terminal nucleotide binding domain. This line was generated using retroviral vectors encoding hOCT3/4, hSOX2, hKLF4 and hcMYC transgenes (Addgene Plasmids #17217, #17218, #17219, #17220 respectively). This line has been stably maintained beyond passage 20, expresses the endogenous stem cell markers such as, OCT4, SOX2, NANOG, SSEA4 and exhibits a normal karyotype, forms teratomas comprising of all three lineage cells. It can efficiently generate highly pigmented and mature retinal pigmented epithelium in 2D adherent cultures and 3D retinal organoids in suspension cultures. This line does not have integrated copies of the cMYC transgene in the genome. |
| User feedback | |
Provider |
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| Generator |
L.V. Prasad Eye Institute (LVPEI)
Contact:
L.V. Prasad Eye Institute (LVPEI) |
| Owner | L.V. Prasad Eye Institute (LVPEI) |
| Distributors | |
External Databases |
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| BioSamples | SAMEA115082726 |
| Cellosaurus | CVCL_D6MV |
| Wikidata | Q127382714 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: allowed
Additional restrictions:
Terms and conditions apply for commercial use |
hIPSC Derivation
General |
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