LRB017

General#

Cell Line

hPSCreg Name KUe014-A
Alternative name(s)
LRB017
Cell line type Human embryonic stem cell (hESC)
Last update 28th July 2017
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Provider

Generator University of Copenhagen (KU)
Derivation country Denmark

External Databases

Cellosaurus CVCL_B250

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Phenotype and Disease related information (Donor)

Diseases

Ethics#

We are currently revising this section.

hESC Derivation#

Date of derivation 2008-03-03
Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
No
Expansion status 3
Stephenson 2007
ICM morphology B
Stephenson 2007
Trophectoderm morphology b
Stephenson 2007
ZP removal technique Mechanical
Cell isolation Mechanical
Cell seeding Embryo

Culture Conditions#

Feeder cells
Yes
Passage method Mechanically
O2 Concentration 6,0 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 15 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3B
Yes
GABRB3
Yes
GDF3
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
TDGF1
Yes

Microbiology / Virus Screening

Genotyping#

Karyotyping (Cell Line)

Other Genotyping (Cell Line)