LRB017
KUe014-A
General
Cell Line |
|
| hPSCreg name | KUe014-A |
| Cite as: | KUe014-A (RRID:CVCL_B250) |
| Alternative name(s) |
LRB017
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines | No similar lines found. |
| Last update | 31st August 2022 |
| User feedback | |
Provider |
|
| Generator |
University of Copenhagen (KU)
Contact:
Laboratory of Reproductive Biology |
| Derivation country | Denmark |
External Databases |
|
| Cellosaurus | CVCL_B250 |
| Wikidata | Q54903026 |
General Information |
|
| Publications | |
| * Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
|
Phenotype and Disease related information (Donor) |
|
| Diseases | |
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | |
| How may genetic information associated with the cell line be accessed? |
hESC Derivation
| Date of derivation | 2008-03-03 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
No |
| Expansion status |
3
|
| ICM morphology |
B
|
| Trophectoderm morphology |
b
|
| ZP removal technique | Mechanical |
| Cell isolation | Mechanical |
| Cell seeding | Embryo |
Culture Conditions
| Feeder cells |
Yes |
| Passage method | Mechanically |
| O2 Concentration | 6,0 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: Knockout-DMEM
Main protein source: Knock-out serum replacement Serum concentration: 15 % |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| DNMT3B |
Yes |
|
||||
| GABRB3 |
Yes |
|
||||
| GDF3 |
Yes |
|
||||
| NANOG |
Yes |
|
||||
| POU5F1 (OCT-4) |
Yes |
|
||||
| TDGF1 |
Yes |
|
Microbiology / Virus Screening |
|
Genotyping
Karyotyping (Cell Line) |
|
Other Genotyping (Cell Line) |
Login to share your feedback, experiences or results with the research community.