Characterization of the human induced pluripotent stem cell (iPSC) SZGJMSi004-A line from a 28-year-old Han male patient with depression
Summary
Peripheral blood mononuclear cells (PBMCs) from a 28-year-old male patient with unipolar depression were reprogrammed with reprogramming factors by electroporation. The pluripotency of transgene-free induced pluripotent stem cells (iPSCs) was verified by immunofluorescence staining for pluripotency markers, and these iPSCs were able to differentiate into the 3 germ layers in vitro. These iPSCs also showed normal karyotypes. Thus, we believe that these iPSCs could be valuable models for exploring the underlying biological mechanism of depression and the safety of antidepressants through the use of iPSCs differentiated into different kinds of neurons or brain organoids. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Yin X, Cai Y, Jia N, Hui L, Zhu Z |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Jun;77:103428 |
| PubMed | 38696853 |
| DOI | 10.1016/j.scr.2024.103428 |