SZGJMSi004-A

WJM-1

General

Cell Line

hPSCreg name SZGJMSi004-A
Cite as:
SZGJMSi004-A (RRID:CVCL_D0M8)
Alternative name(s)
WJM-1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Last update 11th January 2023
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Provider

Generator Research Center of Biological Psychiatry, Suzhou Guangji hospital (SZGJMS)
Owner Research Center of Biological Psychiatry, Suzhou Guangji hospital (SZGJMS)

External Databases

BioSamples SAMEA112294709
Cellosaurus CVCL_D0M8
Wikidata Q123033590

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: allowed
Additional restrictions:

The third parties should sign a biologica material transfer agreement with the Affiliated Guangji Hospital of Soochow University

Donor Information

General Donor Information

Sex male
Ethnicity Han nationality (China)

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Depression
  • Depressivity
  • Depressive episode
  • Depressive disorder
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA112294710

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: xuyuan_yin@163.com
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? the Medical Ethical Committee of the Affiliated Guangji Hospital of Soochow University
Approval number 2019–026
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
A sample of peripheral blood mononuclear cells.
Synonyms
  • Peripheral Blood Mononuclear Cell Specimen
  • PBMC Specimen
  • PBMC Sample
  • Peripheral Blood Mononuclear Cell Sample
  • Peripheral Blood Mononuclear Cell (PBMC)
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
SSEA-4
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 33

Other Genotyping (Cell Line)