Generation of human GAPDH knock-in reporter iPSC lines for stable expression of tdTomato in pluripotent and differentiated culture conditions


Human induced pluripotent stem cells (iPSCs) can differentiate into multiple cell types and are utilized for research on human development and regenerative medicine. Here, we report the establishment of human GAPDH knock-in reporter iPSC lines (GAPDH-tdT1 and 2), via CRISPR/Cas9-mediated homologous recombination, that stably express tdTomato as a constitutive cell label in both iPSCs and their differentiated derivatives. These cell lines will provide useful tools to trace cell locations and fates in 2D cultures and 3D organoids and will facilitate in vivo experiments. Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Kamiya D, Yamashita T, Akaboshi T, Yamaguchi Y, Toyooka Y, Ikeya M
Journal Stem cell research
Publication Date 2022 Apr;60:102704
PubMed 35176664
DOI 10.1016/j.scr.2022.102704

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