Testing of nine different xeno-free culture media for human embryonic stem cell cultures


None of the studied xeno-free media was able to maintain the undifferentiated growth of hESC. The medium containing 20% human serum was found to sustain undifferentiated hESC proliferation to some extent, yet was inferior to the conventional ko-SR-containing medium.

Authors Rajala K, Hakala H, Panula S, Aivio S, Pihlajamäki H, Suuronen R, Hovatta O, Skottman H
Journal Human reproduction (Oxford, England)
Publication Date 2007 May;22(5):1231-8
PubMed 17251355
DOI 10.1093/humrep/del523

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