RC9, RC-9
RCe013-A
General#
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
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Diseases | |
External Databases (Donor) |
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BioSamples | SAMEA2825966 |
Ethics#
We are currently revising this section.hESC Derivation#
Date of derivation | 2009-09-16 |
Embryo stage | Blastula with ICM and Trophoblast |
Supernumerary embryos from IVF treatment? |
Yes
|
PGD Embryo? |
No |
ZP removal technique | Spontaneous Hatching |
Cell isolation | None |
Cell seeding | Embryo |
Derivation under GMP? |
Yes |
Culture Conditions#
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
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SSEA-4 |
Yes |
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TRA 1-60 |
Yes |
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SSEA-1 |
No |
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Differentiation Potency
Microbiology / Virus Screening |
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HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
46XY
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Other Genotyping (Cell Line) |
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