Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC lines for integrating DNA payloads


STRAIGHT-IN is a platform to precisely integrate DNA payloads into the genome of cells, including hiPSCs. Here, we generated two hiPSC acceptor lines each with one copy of an upgraded landing pad (LP). This improved design allows more efficient (∼100 %) and rapid (∼2-3 weeks) generation of genetically modified hiPSC lines containing the desired payloads. This new LP version was inserted into either the AAVS1 (LUMCi004-A-1) or CLYBL (LUMCi004-A-2) safe harbour loci in the hiPSC line, LUMC0099iCTRL04. The resulting lines can be used for the targeted integration of a wide range of transgenes, thereby making them suitable for numerous research applications. Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Blanch-Asensio A, van der Vaart B, Vinagre M, Groen E, Arendzen C, Freund C, Geijsen N, Mummery CL, Davis RP
Journal Stem cell research
Publication Date 2022 Dec 5;66:102991
PubMed 36495703
DOI 10.1016/j.scr.2022.102991

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