LUMCi004-A-2

LU99_CLYBL-bxb-v2

General

Cell Line

hPSCreg Name
LUMCi004-A-2
Alternative name(s)
LU99_CLYBL-bxb-v2
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 28th November 2022
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Provider

Generator Leiden University Medical Center (LUMC)
Owner LUMC hiPSC core facility
Distributors
Derivation country Netherlands

External Databases

BioSamples SAMEA112164541

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclone of

Donor Information

General Donor Information

Sex female
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Is the medical history available upon request? No
Is clinical information available? No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA5032583

Ethics

Also have a look at the ethics information for the parental line LUMCi004-A .
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?
Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? No

hIPSC Derivation

General

The source cell information can be found in the parental cell line LUMCi004-A.

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Laminin
Feeder cells
No
Passage method Enzymatically
TrypLE
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemFlex
Main protein source: Fetal bovine serum
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Differentiation Potency

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal, 46, XX
Passage number: P10

Other Genotyping (Cell Line)

Genetic Modification

Genetic modifications not related to a disease
CLYBL (target)
Transgene expression
citrate lyase beta-like (CLYBL), chromosome 13
TALEN