LU99_AAVS1-bxb-v2
LUMCi004-A-1
General
Cell Line |
|
| hPSCreg name | LUMCi004-A-1 |
| Cite as: | LUMCi004-A-1 (RRID:CVCL_C6UC) |
| Alternative name(s) |
LU99_AAVS1-bxb-v2
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
LUMCi004-A (LUMC0099iCTRL04) MRIi003-A-9 (HK-AAVS1-CAG-FRT-stop-FRT-mKate2-hetero) MRIi003-A-6 (AAVS1-CAG-VSFP-homo) MRIi003-A-5 (AAVS1-CAG-VSFP-hetero) LUMCi004-A-2 (LU99_CLYBL-bxb-v2) LUMCi004-B (LUMC0030iCTRL12) LUMCi004-C (LUMC0031iCTRL08) |
| Last update | 28th November 2022 |
| User feedback | |
Provider |
|
| Generator |
Leiden University Medical Center (LUMC)
Contact:
LUMC hiPSC core facility |
| Owner | LUMC hiPSC core facility |
| Distributors | |
| Derivation country | Netherlands |
External Databases |
|
| BioSamples | SAMEA112164540 |
| Cellosaurus | CVCL_C6UC |
| Wikidata | Q117704634 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | caucasian |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Family history | No |
| Is the medical history available upon request? | No |
| Is clinical information available? | No |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Yes
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA5032583 |
Ethics
Also have a look at the ethics information for the parental line
LUMCi004-A
.
| Is there an MTA available for the cell line? | Yes |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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|
The source cell information can be found in the parental cell line
LUMCi004-A.
|
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Reprogramming method |
|
| Vector type | None |
Vector free reprogramming |
|
| Type of used vector free reprogramming factor(s) |
mRNA
|
Other |
|
| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Laminin |
| Feeder cells |
No |
| Passage method |
Enzymatically
TrypLE
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: StemFlex Medium
Main protein source: Fetal bovine serum Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
Differentiation Potency
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
Normal, 46, XX
Passage number: P10
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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Genetic Modification
| Genetic modifications not related to a disease |
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