CIGLi001-A-2
General
Cell Line |
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| hPSCreg name | CIGLi001-A-2 |
| Cite as: | CIGLi001-A-2 |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 6th December 2024 |
| User feedback | |
Provider |
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| Generator |
Vilnius University (VULSC)
Contact:
Vilnius University (VULSC) |
| Derivation country | Lithuania |
External Databases |
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| BioSamples | SAMEA117481504 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
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| Sex | female |
| Ethnicity | Hispanic |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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| Family history | no |
Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
Yes
Whole genome sequencing
Passes test for quality control as mentioned by supplier vcf File: 802 30F datasheet.pdf The company, SYNTHEGO, that is supplying the cell line to us, mentions that quality control assessment is done and sequence validation data can be provided upon request |
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA114652490 |
Ethics
Also have a look at the ethics information for the parental line
CIGLi001-A
.
| Is there an MTA available for the cell line? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | Plasmids created in this study: Addgene 223321 Addgene 223323 Addgene 223328 |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
CIGLi001-A.
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Reprogramming method |
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Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
mRNA
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Other |
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| Selection criteria for clones | Constitutive GNAT family puromycin N- acetyltransferase (GENE ID: WP_335902160.1) |
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Laminin |
| Feeder cells |
No |
| Passage method |
Enzymatically
Accutase
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| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: Stemfit
Main protein source: Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
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| SSEA-4 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
In vitro directed differentiation
Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 6
Karyotyping method:
Molecular karyotyping by SNP array
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Other Genotyping (Cell Line) |
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| Is there genome-wide genotyping or functional data available? |
Yes
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Genetic Modification
| Genetic modifications not related to a disease |
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