Generation of a genetically encoded voltage indicator MARINA reporter human iPS cell line using Cas9 (VULSCi002-A-2)
Summary
Fluorescent protein-based Genetically Encoded Voltage Indicators (GEVI) offer a remarkable system for high-throughput screening of membrane potential phenotypes. The GEVI MARINA is a derivative from ArcLight, which conversely to ArcLight increases its fluorescence intensity alongside depolarization. Here we created knock-in reporter human iPS cell lines carrying the MARINA reporter using SpCas9 programmable nuclease and characterize a heterozygous clone. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Petruškevičiūtė A, Šimuliūnaitė U, Polanco CM, Rojas B, Kuras S, Valatkaitė-Rakštienė B, Norvilas R, Vijaya AK, Muñoz P, Neniškytė U, Jakubauskas A, Burokas A, Nalvarte I, Inzunza J, Naumovas D, Stoškus M, Griškevičius L, Baltriukienė D, Arias J |
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| Journal | Stem cell research |
| Publication Date | 2025 Feb;82:103628 |
| PubMed | 39721357 |
| DOI | 10.1016/j.scr.2024.103628 |