RPChiPS8023G1, iPSC TBX4 WT, StemRNA™ Human iPSC 802-3G, SYNTHEGO 802-30F
CIGLi001-A
General
Cell Line |
|
| hPSCreg name | CIGLi001-A |
| Cite as: | CIGLi001-A |
| Alternative name(s) |
RPChiPS8023G1, iPSC TBX4 WT, StemRNA™ Human iPSC 802-3G, SYNTHEGO 802-30F
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
CIGLi001-A-1 (SYNTHEGO 802-30F MUT, iPSC TBX4 MUT) |
| Last update | 2nd February 2024 |
| Notes | Synthego supplies the following iPS cell line from an ethically sourced female donor that has been reprogrammed by REPROCELL® from blood-derived endothelial progenitor cells (EPCs) using mRNA (StemRNA™ 3rd-Gen technology) |
| User feedback | |
Provider |
|
| Generator | Center for Infection and Genomics of the Lung (CIGL) |
| Distributors | |
| Derivation country | United States |
External Databases |
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| BioSamples | SAMEA114652059 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Additional restrictions:
SYNTHEGO and REPROCELL which are the suppliers of the cells say - This product is intended for Research Use Only. Commercial Use may require additional agreements. |
| Subclones | |
Donor Information
General Donor Information |
|
| Sex | female |
| Age of donor (at collection) | 30-34 |
| Ethnicity | Hispanic |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes | no phenotypes |
| Family history | no |
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Unknown
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Whole genome sequencing
Passes test for quality control as mentioned by supplier vcf File: 802 30F datasheet.pdf The company, SYNTHEGO, that is supplying the cell line to us, mentions that quality control assessment is done and sequence validation data can be provided upon request |
Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
|
| BioSamples | SAMEA114652490 |
Ethics
| Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
| Was the consent voluntarily given? | Yes |
| Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
| Can you provide us with a copy of the Donor Information Sheet provided to the donor? | Yes |
| Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
| If you do not hold the Donor Consent Form, do you know who does? | Yes |
| Please provide the contact information | Please contact info-us@reprocell.com https://www.reprocell.com/product-catalog/induced-pluripotent-stem-cells/stemrna-human-ipsc-802-3g |
| Is there other documentation provided to the donor for consenting purposes? | No |
| Confirm that consent was obtained by a qualified professional | Yes |
| Has the donor agreed to be re-contacted? | Yes |
| Has the donor been informed about how her/his data will be protected? | Yes |
| Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
| Does consent explicitly allow the derivation of pluripotent stem cells? | Yes |
| Does consent pertain to a specific research project? | Yes |
| Details on restriction to research project | This blood or the components of this blood (plasma, red blood cells, white blood cells, platelets) will be used for a variety of types of research including testing of potential therapeutic agents and evaluation in animal models. |
| Does consent permit unforeseen future research, without further consent? | No |
| Does the consent permit uses of donated embryo/tissue or derived cell line intended for clinical treatment or human applications? | No |
| Does consent expressly prevent development of commercial products? | No |
| Does consent expressly prevent financial gain from any use of the donated embryo/tissue, including any product made from it? | No |
| Does consent expressly permit storage of donated embryo/tissue for an unlimited time? | Yes |
| Does consent expressly permit storage of cells derived from the donated embryo/tissue for an unlimited time? | Yes |
| Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
| Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
Does consent permit research by | |
| an academic institution? | Yes |
| a non-profit company? | Yes |
| Does consent expressly permit collection of genetic information? | Yes |
| Does consent expressly permit storage of genetic information? | Yes |
| Has the donor been informed that their donated biosample or derived cells may be tested for the presence of microbiological agents / pathogens? | Yes |
| Has the donor consented to receive information discovered during use of donated embryo/tissue that has significant health implications for the donor? | Yes |
| How may genetic information associated with the cell line be accessed? | Controlled Access |
| Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
| Does the consent anticipate that the donor will be notified of results or outcomes of any research involving the donated samples or derived cells? | No |
| Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? | No |
| Does the consent permit the donor, upon withdrawal of consent, to stop delivery or use of information and data about the donor? | Yes |
| Does consent permit access to medical records of the donor? | Yes |
| Please describe how access is provided: | The following individuals may see the medical records, and possibly record personal health information about the donor: authorized employees of Research Blood Components, LLC, employees of the FDA or other authorized state or federal agencies, and representatives of the New England Institutional Review Board. Federal law provides that donor's doctor can only share your health information with the people the donor has permitted in this form. Once they receive your health information, those people may share it with other people not listed on this form (however donor name and other personal identifiers will not be disclosed). |
| Does consent permit access to any other source of information about the clinical treatment or health of the donor? | Yes |
| Contact data, institution, or website: | FDA - de-identified information |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | New England Independent Review Board |
| Approval number | #04-144 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? | Yes |
| Name of accrediting authority involved? | New England Independent Review Board |
| Approval number | #04-144 |
| Do you have obligations to third parties in regard to the use of the cell line? | No |
| Are you aware of any further constraints on the use of the donated embryo/tissue or derived cells? | No |
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? | REPROCELL |
| Are you aware of any constraints on the use or distribution of the cell line from the owner or any parties identified in the query above? | No |
hIPSC Derivation
General |
|
| Source cell line name | Peripheral blood-derived endothelial progenitor cells (EPCs) |
| Source cell type (free text) | Peripheral blood-derived endothelial progenitor cells (EPCs) |
| Age of donor (at collection) | 30-34 |
| Collected in | 2015 |
| Source cell line vendor | REPROCELL |
| Passage number reprogrammed | 14 |
Reprogramming method |
|
| Vector type | None |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
mRNA, miRNA
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| mRNA | |
Other |
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| Selection criteria for clones | Day 6, 8, 13 primary EPC-RNA-iPSC colonies were identified using Stemgent StainAlive TRA-1-60 antibody and isolated from the primary culture by Day 12-14 |
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
Yes |
| Available as clinical grade? |
Unknown |
Culture Conditions
| Surface coating | iMatrix added in medium | ||||||
| Feeder cells |
No |
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| Passage method |
Enzymatically
Accutase
|
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| O2 Concentration | 21 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
Other medium:
Base medium: StemMACS™ iPS-Brew XF, human
Main protein source: Serum concentration: 0 % Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
||||||
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Unknown |
||||||
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| SSEA-4 |
Yes |
|||||
| TRA 1-60 |
Yes |
| Marker | Present | Absent |
| mCpG | ||
| OCT4 | X |
Report
802 30F datasheet.pdf
Figure 4 - Oct4 expression
Tests for iPSC-REPROCELL StemRNA-Human-iPSCs_US.pdf
Oct4 expression in first figure
| Pluripotency Score | Novelty Score | |
| 37.18372 | 1.282409 |
Report
802 30F datasheet.pdf
Table 1
Morphology pictures
802 30F datasheet.pdf
Figure 4 - The cells growing in colonies
Self-renewal
Unknown
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Data analysis report
Tests for iPSC-REPROCELL StemRNA-Human-iPSCs_US.pdf
The cells differentiate into all 3 germ layers
Differentiation Potency
Gut epithelium
Morphology
Tests for iPSC-REPROCELL StemRNA-Human-iPSCs_US.pdf
Page 2 - StemRNA human iPSCs differentiate in vivo into all three germ layers
Gut connective tissue
Morphology
Tests for iPSC-REPROCELL StemRNA-Human-iPSCs_US.pdf
Page 2 - StemRNA human iPSCs differentiate in vivo into all three germ layers
Epidermis and hair follicle
Morphology
Tests for iPSC-REPROCELL StemRNA-Human-iPSCs_US.pdf
Page 2 - StemRNA human iPSCs differentiate in vivo into all three germ layers
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Certificate of Analysis |
|
| Is there a certificate of analysis available? |
Yes
Passage:
P19
|
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46, XX
Passage number: P14
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|
| Is there genome-wide genotyping or functional data available? |
Yes
Whole genome sequencing
Passes test for quality control as mentioned by supplier vcf File: 802 30F datasheet.pdf The company, SYNTHEGO, that is supplying the cell line to us, mentions that quality control assessment is done and sequence validation data can be provided upon request Tests for iPSC-REPROCELL StemRNA-Human-iPSCs_US.pdf The company, REPROCELL; that supplies the cell line confirmed in this document that they did whole genome sequencing and that it passes the test
SNP typing array
KaryoStat+ indicates 802-30F cells are genomically stable vcf File: 802 30F datasheet.pdf Figure 1. KaryoStat+ indicates 802-30F cells are genomically stable.* KaryoStat+ results for wild type 802-30F cells (passage 19) indicate that genomic integrity is maintained. The whole-genome view displays all somatic and sex chromosomes in one frame with a high-level copy number. The smooth signal plot (right y-axis) is the smoothing of the log2 ratios which depict the signal intensities of probes on the microarray. A value of 2 represents a normal copy number state (CN = 2). A value of 3 represents chromosomal gain (CN = 3). A value of 1 represents a chromosomal loss (CN = 1). The pink, green and yellow colors indicate the raw signal for each individual chromosome probe, while the blue signal represents the normalized probe signal which is used to identify copy number and aberrations (if any). * descriptions adapted from Thermo Fisher Scientific. |
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