Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4(+)T cells of healthy individuals of Indian origin


Human induced pluripotent stem cell-lines (iPSCs) of Indian origin NCCSi005A and NCCSi006A were established by reprogramming of CD4+T cells, isolated from the peripheral blood mononuclear cells (PBMCs) of two healthy female donors. Reprogramming was achieved using integration free, Sendai viral vector system expressing cocktail of transcription factors KOS, hc-MYC and hKLF4. Both the established cell-lines showed alkaline phosphatase activity, expressed stemness markers, exhibited normal female karyotype and displayed potential for tri-lineage differentiation. These two CD4+T cells derived cell-lines represent valuable resource as control iPSC cell lines of Indian origin. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Khan M, Zende S, Vaidyanath A, Avatade R, Shiras A
Journal Stem cell research
Publication Date 2019 Aug;39:101506
PubMed 31352200
DOI 10.1016/j.scr.2019.101506

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