Biallelic and monoallelic deletion of the RB1 promoter in six isogenic clonal H9 hESC lines


Retinoblastoma is a childhood tumor of the retina that is caused mostly by biallelic inactivation of the tumor suppressor gene RB1. To generate a research resource, we abrogated expression of RB1 in H9 hESCs by CRISPR/Cas9 induced deletion of the RB1 promoter, either on one or on both alleles. This enables studies on the role of RB1 loss during differentiation, for example in differentiation towards neural retina. The generation of three isogenic lines per deletion state enables validation of phenotypic results in independent clonal lines. Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Döpper H, Horstmann M, Menges J, Bozet M, Kanber D, Steenpass L
Journal Stem cell research
Publication Date 2020 May;45:101779
PubMed 32268247
DOI 10.1016/j.scr.2020.101779

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