General

Cell Line

hPSCreg Name
XACHi004-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 7th November 2022
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Provider

Generator Xi'an children's hospital (XACH)

External Databases

Cellosaurus CVCL_YM77
Wikidata Q98135109
BioSamples SAMEA111507229
CLO CLO_0104153

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Cell line can only be used in: research clinical use
Research use: allowed
Clinical use: allowed
Commercial use: allowed
Additional restrictions:

no

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Atrial Fibrillation
Synonyms
  • AFib
  • AF
  • Atrial fibrillation
  • Atrial Fibrillation
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes

External Databases (Donor)

BioSamples SAMEA111507228

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? the Ethics Committee of Xi’an Children’s Hospital, Affiliated Children’s Hospital of Xi’an Jiaotong University.
Approval number (No. 2019-599)
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Derived Peripheral Blood Mononuclear Cell
Mononuclear cells collected from peripheral blood.
Synonyms
  • Peripheral Blood Mononuclear cells
  • Derived Peripheral Blood Mononuclear Cell

Reprogramming method

Vector type Integrating
Vector Virus ()
Is the used vector excisable?
Unknown
Absence of reprogramming vector(s)?
Unknown
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Marker Present Absent
mCpG X
OCT4 X
Morphology pictures
Self-renewal
Positive
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Data analysis report
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Marker Expressed
AFP
Yes
Mesoderm
Ont Id: UBERON_0000926
Marker Expressed
TBX1
Yes
Ectoderm
Ont Id: UBERON_0000924
Marker Expressed
PAX6
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46
Passage number: 40
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes