ZZUNEUi026-A
General
Cell Line |
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hPSCreg name | ZZUNEUi026-A |
Cite as: | ZZUNEUi026-A (RRID:CVCL_B7T1) |
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
UKKi035-A (NP0139-A, NP0139-3E) Donor's gene variants: MYBPC3, MYBPC3 Donor diseases: Rare hypertrophic cardiomyopathy UKKi035-B (NP0139-B, NP0139-6C) Donor's gene variants: MYBPC3, MYBPC3 Donor diseases: Rare hypertrophic cardiomyopathy UKKi035-C (NP0139-C, NP0139-24D) Donor's gene variants: MYBPC3, MYBPC3 Donor diseases: Rare hypertrophic cardiomyopathy SCVIi074-A (SCVIi599C1) Donor's gene variants: BAG cochaperone 3 Donor diseases: Hypertrophic Cardiomyopathy |
Last update | 12th May 2022 |
User feedback | |
Provider |
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Generator | ZhengZhou University- The first affiliated hospital(ZZU) (ZZUNEU) |
Owner | ZhengZhou University- The first affiliated hospital(ZZU) (ZZUNEU) |
Distributors | |
Derivation country | China |
External Databases |
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BioSamples | SAMEA14300442 |
Cellosaurus | CVCL_B7T1 |
Wikidata | Q112930600 |
General Information |
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Publications | |
* Is the cell line readily obtainable for third parties? |
No |
Donor Information
General Donor Information |
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Sex | male |
Ethnicity | Han |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Family history | None |
Is the medical history available upon request? | Yes |
Is clinical information available? | Yes |
Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Yes
Karyotyping method:
G-Banding
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
Yes
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External Databases (Donor) |
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BioSamples | SAMEA14300441 |
Ethics
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? | Yes |
Was the consent voluntarily given? | Yes |
Has the donor been informed that participation will not directly influence their personal treatment? | Yes |
Can you provide us with a copy of the Donor Information Sheet provided to the donor? | No |
Please provide contact information of the holder of the original Donor Information Sheet. | inly163@163.com |
Do you (Depositor/Provider) hold the original Donor Consent Form? | No |
If you do not hold the Donor Consent Form, do you know who does? | Yes |
Please provide the contact information | inly163@163.com |
Alternatives to consent are available? | No |
Is there other documentation provided to the donor for consenting purposes? | No |
Confirm that consent was obtained by a qualified professional | Yes |
Has the donor agreed to be re-contacted? | Yes |
Has the donor been informed about how her/his data will be protected? | Yes |
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. | anonymised |
Does consent explicitly allow the derivation of pluripotent stem cells? | No |
Does consent expressly prevent the derivation of pluripotent stem cells? | No |
Does consent pertain to a specific research project? | No |
Does consent permit unforeseen future research, without further consent? | Yes |
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? | No |
How may genetic information associated with the cell line be accessed? | Controlled Access |
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? | No |
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
Name of accrediting authority involved? | Ethics Committee of the Frist Affiliated Hospital of Zhengzhou University |
Approval number | 2018-KY-38 |
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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Source cell type |
A sample of peripheral blood mononuclear cells.
Synonyms
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Reprogramming method |
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Vector type | Non-integrating |
Vector | Sendai virus |
Is reprogramming vector detectable? |
No |
Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
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O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ Plus
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
Differentiation Potency
In vitro directed differentiation
Genotyping
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
G-banding karyotyping(46, XY)
Passage number: 15
Karyotyping method:
G-Banding
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Other Genotyping (Cell Line) |
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