ZZUNEUi026-A

General

Cell Line

hPSCreg Name
ZZUNEUi026-A
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 12th May 2022
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Provider

Generator ZhengZhou University- The first affiliated hospital(ZZU) (ZZUNEU)
Owner ZhengZhou University- The first affiliated hospital(ZZU) (ZZUNEU)
Distributors
Derivation country China

External Databases

BioSamples SAMEA14300442
CLO CLO_0103860
Cellosaurus CVCL_B7T1
Wikidata Q112930600

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Ethnicity Han

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Hypertrophic cardiomyopathy
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • HCM
  • Cardiomyopathy, hypertrophic
  • Enlarged and thickened heart muscle
Family history None
Is the medical history available upon request? Yes
Is clinical information available? Yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes

External Databases (Donor)

BioSamples SAMEA14300441

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Provide contact information of the holder of the original Donor Information Sheet: inly163@163.com
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink inly163@163.com
Alternatives to consent are available? No
Is there other documentation provided to the donor for consenting purposes? No
Confirm that consent was obtained by a qualified professional Yes
Has the donor agreed to be re-contacted? Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent permit unforeseen future research, without further consent? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethics Committee of the Frist Affiliated Hospital of Zhengzhou University
Approval number 2018-KY-38
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Peripheral Blood Mononuclear Cell Sample
A sample of peripheral blood mononuclear cells.
Synonyms
  • Peripheral Blood Mononuclear Cell Specimen
  • PBMC Specimen
  • PBMC Sample
  • PBMC
  • Peripheral Blood Mononuclear Cell Sample
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Differentiation Potency
Hepatocyte
Ont Id: CL_0000182
In vitro directed differentiation
Marker Expressed
RBFOX2
Yes
alpha fetoprotein
Yes
Morphology
Smooth Muscle Cell
Ont Id: CL_0000192
In vitro directed differentiation
Marker Expressed
ACTA2
Yes
NKX2-5
Yes
Morphology
Neuron
Ont Id: CL_0000540
In vitro directed differentiation
Marker Expressed
β3-Tubulin
Yes
NeuN
Yes
Morphology

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
G-banding karyotyping(46, XY)
Passage number: 15
Karyotyping method: G-Banding

Other Genotyping (Cell Line)