NP0075-11B

General#

Cell Line

hPSCreg Name UKKi017-C
Alternative name(s)
NP0075-11B
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator Klinikum der Universität zu Köln (UKK)
Owner Institute for Neurophysiology, Medical Faculty
Distributors
Derivation country Germany

External Databases

BioSamples SAMEA17621668
Cellosaurus CVCL_LD24
ECACC 66540477
EBiSC UKKi017-C

General Information

Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex female
Ethnicity German

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
hypertrophic cardiomyopathy
Genetic testing of the patient was not performed
The donor is affected.
Synonyms
  • Asymmetric Septal Hypertrophies
  • Hypertrophic Obstructive Cardiomyopathies
  • Cardiomyopathy, hypertrophic
  • Subvalvular Stenosis, Idiopathic Hypertrophic
  • Hypertrophic Obstructive Cardiomyopathy
  • HYPERTR OBSTR CARDIOMYOP
  • hyper. obst. cardiomyopathy
  • SUBVALV STENOSIS
  • HCM - Hypertrophic cardiomyopathy
  • IDIOPATHIC HYPERTROPHIC SUBVALV STENOSIS
  • Obstructive Cardiomyopathy, Hypertrophic
  • Hypertrophies, Asymmetric Septal
  • Hypertrophic Cardiomyopathies
  • Cardiomyopathies, Hypertrophic Obstructive
  • Hypertrophic cardiomyopathy (disorder)
  • HOCM - Hypertrophic obstructive cardiomyopathy
  • Primary hypertrophic cardiomyopathy
  • Idiopathic Hypertrophic Subvalvular Stenosis
  • IHSS
  • primary hypertrophic cardiomyopathy (disorder) [Ambiguous]
  • Idiopathic Hypertrophic Subaortic Stenosis
  • Obstructive cardiomyopathy
  • SUBVALV STENOSIS IDIOPATHIC HYPERTROPHIC
  • Hypertrophy, Asymmetric Septal
  • Hypertrophic obstructive cardiomyopathy (disorder)
  • Septal Hypertrophy, Asymmetric
  • Cardiomyopathies, Hypertrophic
  • IHSSs
  • hypertrophic myocardiopathy
  • Cardiomyopathy, Hypertrophic Obstructive
  • HCM
  • Obstructive Cardiomyopathies, Hypertrophic
  • Asymmetric Septal Hypertrophy
  • Septal Hypertrophies, Asymmetric
show more synonyms
Is the medical history available upon request? yes
Is clinical information available? yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA17620168

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
peripheral blood mononuclear cell
A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.
Age of donor (at collection) 50-54
Collected in 2016
Passage number reprogrammed 0

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection sendai virus absent
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™
Supplements
Essential 8 supplement 50x 10 ml
Essential 8 Flex supplement 50x 10 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
DNMT3b
Yes
FOXD3
Yes
NANOG
Yes
OCT 4
Yes
SOX2
Yes
SSEA-1
Yes
SSEA-4
Yes
TRA 1-80
Yes
CD90
Yes
Morphology pictures
morphology pictures of iPS cell line NP0075-11B before banking and of the thawing control
morphology pictures of iPS cell line NP0075-11B before banking and of the thawing control
morphology pictures of iPS cell line NP0075-11B before banking and of the thawing control
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Marker Expressed
SOX17
Yes
Morphology
expression of SOX17 in differentiated iPS cell line NP0075-11B
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
BRACHYURY
Yes
Morphology
expression of BRACHYURY in differentiated iPS cell line NP0075-11B
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation
Marker Expressed
Nestin
Yes
Morphology
expression of NESTIN in differentiated iPS cell line NP0075-11B

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No larger chromosomal aberrations observed
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)