GRCHJUi001-A

LYJ-iPS-C

General

Cell Line

hPSCreg name GRCHJUi001-A
Cite as:
GRCHJUi001-A
Alternative name(s)
LYJ-iPS-C
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines
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(SHOX2 c.*28T>C_isoWT)
Donor diseases:
Atrial Fibrillation
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(Shox2.2)
Donor diseases:
Atrial Fibrillation
MRIi016-A
(Shox2.1)
Donor diseases:
Atrial Fibrillation
ESi108-A
(AFib-C53)
Donor diseases:
Atrial Fibrillation
Last update 25th December 2023
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Provider

Generator Guangzhou Red Cross Hospital of Jinan University (GRCHJU)
Owner Guangzhou Red Cross Hospital of Jinan University (GRCHJU)
Distributors
Derivation country China

External Databases

BioSamples SAMEA115049286

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Ethnicity Chinese Han

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • AFib
  • AF
  • Atrial fibrillation
  • Atrial Fibrillation
show more synonyms
Disease associated phenotypes
Family history atrial fibrillaton
Is the medical history available upon request? Yes
Is clinical information available? Yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA115049287

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? No
Please provide contact information of the holder of the original Donor Information Sheet. shaohengzh67@163.com
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? the Ethics Committee of Hainan General Hospital
Approval number 2022-238
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name urine cells
Derived from same source line (potentially other lot and donor, see below):
Source cell type
The fluid that is excreted by the kidneys. It is stored in the bladder and discharged through the urethra.
Synonyms
  • Urine
  • Urinary System, Urine
  • urine
  • URINE
show more synonyms
Source cell origin
The fluid that is excreted by the kidneys. It is stored in the bladder and discharged through the urethra.
Synonyms
  • Urine
  • Urinary System, Urine
  • urine
  • URINE
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
PCR, Sequencing

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XY
Passage number: 2
Karyotyping method: G-Banding

Other Genotyping (Cell Line)