A prime editor efficiently repaired human induced pluripotent stem cells with AR gene mutation (c.2710G > A; p. V904M)


Prime Editor (PE) is a precise genome manipulation technology based on the CRISPR-Cas9 system, while its application in human induced pluripotent stem cells (iPSCs) remains limited. Here, we established a repaired hiPS cell line (SKLRMi001-A-1) from hiPSCs with androgen receptor (AR) mutation (c.2710G > A; p.V904M). The repaired iPSC line expressed pluripotency markers, retained normal karyotype, showed the capability of differentiating into three germ layers and was absence of mycoplasma infection. The repaired iPSC line will help to elucidate the mechanism of androgen insensitivity syndrome (AIS) and benefit treatment for AIS in the future. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Sun R, Cui Y, Liu Z, Guo J, Zhang X, Zhu P, Sha J, Yang X, Yuan Y
Journal Stem cell research
Publication Date 2023 Jun;69:103102
PubMed 37148822
DOI 10.1016/j.scr.2023.103102

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