Establishment of human embryonic stem cell (SKLRMe001-A)carrying Azoospermic factor c (AZFc) deletions


The absence of azoospermia factor c (AZFc) is a common molecular cause of sperm failure. In men with non-obstructive azoospermia or severe oligospermia, the incidence of AZFc is around 10%. The AZFc region is located at the far end of the Yqll chromosome which has three non-overlapping sub-regions with a high frequency of deletion. Now, we generated a human embryonic stem cell line (SKLRMe001-A) that carries a deleted AZFc gene on the Y chromosome. The ESC line maintains a stem cell-like morphology, pluripotency, and has a normal karyotype. The cells can also differentiate into all three germ layers in vivo. Copyright © 2021 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Li C, Cai L, Dong J, Ning S, Zhou J, Wu H, Cui Y, Qin L, Yang X
Journal Stem cell research
Publication Date 2021 Dec 18;59:102625
PubMed 34952435
DOI 10.1016/j.scr.2021.102625

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