PKU1

General

Cell Line

hPSCreg name BJMUe001-A
Cite as:
BJMUe001-A (RRID:CVCL_C065)
Alternative name(s)
PKU1
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 22nd May 2019
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Provider

Generator Peking University Third Hospital (BJMU)
Derivation country China

External Databases

Cellosaurus CVCL_C065
Wikidata Q54947477

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

Ethics

Alternatives to consent

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
ZP removal technique Enzymatic

Culture Conditions

Feeder cells Mouse embryonic Fibroblasts
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
Medium Other medium:
Base medium: Knockout-DMEM
Main protein source: Fetal bovine serum
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-1
No
SSEA-3
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)