An integration-free iPSC line SDQLCHi065-A from a patient with down syndrome, possessing a 47, XY,+21, inv(9)(p12q21),16qh + karyotype
Summary
Down syndrome, a chromosomal aneuploidy genetic disorder, is primarily caused by trisomy 21 in all cells of a patient's body. In fewer cases, it can be attributed to a trisomy 21 chimera or trisomy 21 in specific cells within the body. We established an induced pluripotent stem cell (iPSC) line from the peripheral blood mononuclear cells (PBMCs) of an 8-day-old boy with Down syndrome possessing a 47, XY,+21, inv(9)(p12q21),16qh + karyotype. The iPSCs exhibited consistent karyotype, expressed markers indicative of pluripotency, lacked genomic integration of episomal plasmids, and demonstrated in vitro differentiation potential across three germ layers. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Zhang X, Xin H, Liu Y, Gai Z, Li Z |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Apr;76:103351 |
| PubMed | 38377649 |
| DOI | 10.1016/j.scr.2024.103351 |