Induced pluripotent stem cell (iPSC) line MLi005-A derived from a patient with dominant dystrophic epidermolysis bullosa (DDEB)
Summary
We have generated MLi005-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a male patient with dominant dystrophic epidermolysis bullosa (DDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi005-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, pluripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi-005A line has been also tested for ability to differentiate into fibroblasts and keratinocytes and markers associated with these cell types. Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.
| Authors | Ropret S, Khurana P, Fabčič T, Cvetkovska J, Trobec A, Jokhadar ŠZ, Ilic D, McGrath JA, Guttmann-Gruber C, Liovic M |
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| Journal | Stem cell research |
| Publication Date | 2024 Mar;75:103306 |
| PubMed | 38271763 |
| DOI | 10.1016/j.scr.2024.103306 |