Generation of 2 induced pluripotent stem cell lines derived from patients with Parkinson's disease carrying LRRK2 G2385R variant


Leucine rich repeat kinase (LRRK2) is the most prevalent genetic cause for Parkinson's disease. LRRK2 p.G2385R is an Asian specific genetic risk factor for sporadic Parkinson's disease. We generated two induced pluripotent stem cells (iPSCs), IBMS-iPSC-018-09 and IBMS-iPSC-020-01, from the peripheral blood mononuclear cells of two patients carrying LRRK2 p.G2385R variant by using the Sendai-virus delivery system. These iPSCs had a normal karyotype and exhibited pluripotency, such as an embryonic stem cell-like morphology, expression of pluripotent markers, and capacity to differentiate into three germ layers. This cellular model will provide a platform for pathophysiological studies of neurodegeneration in Parkinson's disease. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.

Authors Cheng YC, Huang CY, Ho MC, Hsu YH, Syu SH, Lu HE, Lin HI, Lin CH, Hsieh PCH
Journal Stem cell research
Publication Date 2018 Apr;28:1-5
PubMed 29414410
DOI 10.1016/j.scr.2018.01.034

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