ND1C5

General#

Cell Line

hPSCreg Name NUIGi039-B
Alternative name(s)
ND1C5
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 2nd December 2019
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Provider

Generator National University of Ireland Galway (NUIG)

External Databases

BioSamples SAMEA6456110

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: allowed
Commercial: allowed

Donor Information#

General Donor Information

Sex male
Ethnicity caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
autism spectrum disorder
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Development Disorders, Pervasive
  • autistic spectrum disorder
  • Pervasive Child Development Disorders
  • Autism Spectrum Disorders
  • Spectrum Disorders, Autism
  • Disorder, Pervasive Development
  • CHILD DEVELOPMENT DIS PERVASIVE
  • pervasive developmental disorders
  • Development Disorder, Pervasive
  • Disorders, Autism Spectrum
  • Disorder, Autism Spectrum
  • Pervasive Development Disorder
  • autism spectrum disease
  • PERVASIVE CHILD DEVELOPMENT DIS
  • Disorders, Pervasive Development
  • Child Development Disorders, Pervasive
  • autism spectrum disorder
  • Pervasive Development Disorders
  • Spectrum Disorder, Autism
show more synonyms

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA6456099

Ethics#

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold a copy of the SIGNED Donor Consent Form? Yes
If you do not hold the SIGNED Donor Consent Form, have you obtained a copy of the unsigned Donor Consent Form from the holder? No
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Open Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Research Ethics Committee of Galway University Hospitals
Approval number C.A.750
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Research Ethics Committee of Galway University Hospitals
Approval number C.A.750
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation#

General

Source cell type
Fibroblast
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
Gentle Cell Dissociation Reagent
CO2 Concentration 5 %
Medium Essential 8™ Flex

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
Morphology pictures
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Morphology
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Morphology

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
SNP GENOTYPE
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)