NDD05, hiPSC_11080405_c1

General

Cell Line

hPSCreg Name
QBRIi016-A
Alternative name(s)
NDD05, hiPSC_11080405_c1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 2nd October 2022
User feedback
No feedback available yet.

Login to share your feedback, experiences or results with the research community.

Provider

Generator Qatar Biomedical Research Institute (QBRI)

External Databases

Cellosaurus CVCL_C1VX
BioSamples SAMEA111396557
CLO CLO_0104073

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
autism spectrum disorder
Synonyms
  • Development Disorders, Pervasive
  • autistic spectrum disorder
  • Pervasive Child Development Disorders
  • Autism Spectrum Disorders
  • Spectrum Disorders, Autism
  • Disorder, Pervasive Development
  • CHILD DEVELOPMENT DIS PERVASIVE
  • pervasive developmental disorders
  • Development Disorder, Pervasive
  • Disorders, Autism Spectrum
  • Disorder, Autism Spectrum
  • Pervasive Development Disorder
  • autism spectrum disease
  • PERVASIVE CHILD DEVELOPMENT DIS
  • Disorders, Pervasive Development
  • Child Development Disorders, Pervasive
  • autism spectrum disorder
  • Pervasive Development Disorders
  • Spectrum Disorder, Autism
  • pervasive developmental disorder - not otherwise specified
  • PDD
show more synonyms

External Databases (Donor)

BioSamples SAMEA111396558

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Contact information / weblink falshaban@hbku.edu.qa
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Qatar Biomedical Research Institute
Approval number QBRI-IRB 2018-024
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Peripheral Blood Mononuclear Cell
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
  • PERIPHERAL BLOOD MONONUCLEAR CELL
  • Peripheral Blood Mononuclear Cell
  • Peripheral Blood Mononuclear cells
  • PBMC
show more synonyms

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Medium mTeSR™ 1

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
SSEA-4
Yes
TRA 1-60
Yes
TRA 1-81
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
sox17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
Brachury
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
MAP2
Yes

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY[cp20]

Other Genotyping (Cell Line)