Generation of heterozygous (MCRIi030-A-1) and homozygous (MCRIi030-A-2) NR2F2/COUP-TFII knockout human iPSC lines
Summary
The NR2F2 gene encodes the transcription factor COUP-TFII, which is upregulated in embryonic mesoderm. Heterozygous variants in NR2F2 cause a spectrum of congenital anomalies including cardiac and gonadal phenotypes. We generated heterozygous (MCRIi030-A-1) and homozygous (MCRIi030-A-2) NR2F2-knockout induced pluripotent stem cell (iPSC) lines from human fibroblasts using a one-step protocol for CRISPR/Cas9 gene-editing and episomal-based reprogramming. Both iPSC lines exhibited a normal karyotype, typical pluripotent cell morphology, pluripotency marker expression, and the capacity to differentiate into the three embryonic germ layers. These lines will allow us to explore the role of NR2F2 during development and disease. Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.
| Authors | Ferreira LGA, Cabral-da-Silva MC, Pachernegg S, van den Bergen JA, Robevska G, Vlahos K, Howden SE, Ng ES, Dias-da-Silva MR, Sinclair AH, Ayers KL |
|---|---|
| Journal | Stem cell research |
| Publication Date | 2024 Apr;76:103374 |
| PubMed | 38458031 |
| DOI | 10.1016/j.scr.2024.103374 |