CBRCULi015-A

GM23375-1, GM23375 clone 1

General

Cell Line

hPSCreg name CBRCULi015-A
Cite as:
CBRCULi015-A (RRID:CVCL_C9J3)
Alternative name(s)
GM23375-1, GM23375 clone 1
Cell line type Human induced pluripotent stem cell (hiPSC)
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Last update 19th January 2024
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Provider

Generator CERVO Brain Research Centre-Université Laval (CBRCUL)
Derivation country Canada

External Databases

Cellosaurus CVCL_C9J3
BioSamples SAMEA114860034
Wikidata Q123030873

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information

General Donor Information

Sex male
Ethnicity Caucasian, Iraqi

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
The donor is a carrier of a disease-associated mutation and affected.
Synonyms
  • Congenital Myotonic Dystrophy
Genetic variants
DMPK – DM1 protein kinase (target)
19q13.3
Heterozygous
Donor subject has one normal CTG repeat allele and one allele with a CTG repeat size of approximately 1700-1800 in the DMPK gene
Disease associated phenotypes
  • Needed ankle brace at age 1
  • Needed respiratory support for four weeks after birth
  • Difficulty swallowing;
  • Facial weakness
Family history The donor have a clinically affected mother.
Is clinical information available? Donor subject has one normal CTG repeat allele and one allele with a CTG repeat size of approximately 1700-1800 in the DMPK gene

External Databases (Donor)

BioSamples SAMN00806757

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? No
If you do not hold the Donor Consent Form, do you know who does? Yes
Please provide the contact information https://www.coriell.org/
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. pseudonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent pertain to a specific research project? No
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No

Does consent permit research by

an academic institution? Yes
How may genetic information associated with the cell line be accessed? No information
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Does the consent permit the donor, upon withdrawal of consent, to stop the use of the derived cell line(s) that have already been created from donated samples? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved?
Approval number
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved?
Approval number
Is there an MTA available for the cell line? Yes
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell line name GM23375
Source cell type
Source cell line vendor Coriell Institute for Medical Research

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Is reprogramming vector detectable?
No
Methods used
PCR
Notes on reprogramming vector detection The reprogramming vector is not detectable at passage 30.
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
Medium mTeSR™ Plus

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
POU5F1 (OCT-4)
Yes
SOX2
Yes
DNMT3B
Yes
TERT
Yes
REXO1
Yes
Morphology pictures
CBRCULi15-A morphology
Self-renewal
Negative
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Scorecard
Scorecard_CBRCULi015-A.jpg
Analysis of trilineage differentiation potential of CBRCULi015-A with the hPSC Scorecard
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
Scorecard
Mesoderm
Ont Id: UBERON_0000926
Scorecard
Ectoderm
Ont Id: UBERON_0000924
Scorecard

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
XY normal
Passage number: P6
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)