General

Cell Line
hPSCreg name	RGIe057-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	RGIe057-A (RRID:CVCL_B572)
Alternative name(s)	SI-148
Cell line type	Human embryonic stem cell (hESC)
Similar lines	CBRCULi002-A (19-1035-10) Donor diseases: myotonic dystrophy type 1 CBRCULi007-A (14-0730-1) Donor diseases: myotonic dystrophy type 1 CBRCULi004-A (79-1660-2) Donor diseases: myotonic dystrophy type 1 CBRCULi005-A (91-3158-1) Donor diseases: myotonic dystrophy type 1 CBRCULi015-A (GM23375-1, GM23375 clone 1) Donor's gene variants: DMPK – DM1 protein kinase, DMPK – DM1 protein kinase Donor diseases: Congenital Myotonic Dystrophy ZZUi015-A (ZZU-iPS-DM1-002) Donor diseases: myotonic dystrophy type 1 ZZUi006-A (ZZU-iPS-DM1-001) Donor's gene variants: DMPK Donor diseases: Steinert myotonic dystrophy CHUQi001-A (522-2666-2, SAMEA104271492) Donor diseases: myotonic dystrophy type 1 CBRCULi003-A (59-0944-2) Donor diseases: myotonic dystrophy type 1 HADe006-A (HAD 6) Donor diseases: myotonic dystrophy type 1 CENSOi008-A (FB77R2c3, CENSOi260) Donor's gene variants: DMPK, DMPK Donor diseases: myotonic dystrophy type 1 RGIe061-A (SI-153) Donor diseases: myotonic dystrophy type 1 CBRCULi018-A (91-2032-7) Donor diseases: myotonic dystrophy type 1 CBRCULi019-A (19-1741-2) Donor diseases: myotonic dystrophy type 1 CBRCULi020-A (79-1761-15) Donor diseases: myotonic dystrophy type 1 CBRCULi012-A (GM04601 clone 21, GM04601-21) Donor diseases: myotonic dystrophy type 1 CBRCULi013-A (GM04602-2, GM04602 clone 2) Donor diseases: myotonic dystrophy type 1 VUBe003-A (VUB03_DM1) Donor diseases: myotonic dystrophy type 1 KCLe014-A (KCL018_MD1) Donor diseases: myotonic dystrophy type 1 VUBe019-A (VUB19_DM1) Donor diseases: Steinert myotonic dystrophy HADe001-A (HAD 1) Donor diseases: myotonic dystrophy type 1
Last update	3rd September 2022
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Provider
Generator	Reproductive Genetics Institute (RGI) Contact: Stem Cell Research Unit
Derivation country	United States
External Databases
Cellosaurus	CVCL_B572
Wikidata	Q54949895
General Information
Publications	Verlinsky Y et al. Human embryonic stem cell lines with genetic disorders. Reproductive biomedicine online. 2005 Jan;10(1):105-10.
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Sex	male
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Myotonic dystrophy type 1 The donor is affected. Synonyms Dystrophia myotonica Steinert disease congenital myotonic dystrophy myotonic dystrophy of Steinert show more synonyms show less synonyms

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation

Supernumerary embryos from IVF treatment?	Yes
PGD Embryo?	Yes

Culture Conditions

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes
POU5F1 (OCT-4)	Yes
SSEA-3	Yes
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes
TRA 2-39	Yes

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XY Passage number: 7
Other Genotyping (Cell Line)