iPS23-00086 clE2, fIPS_DM1Rescue_P1 clE2
The cell line is not validated yet.
SCTCi043-A-1
General
Cell Line |
|
| hPSCreg name | SCTCi043-A-1 |
| Cite as: | SCTCi043-A-1 |
| Alternative name(s) |
iPS23-00086 clE2, fIPS_DM1Rescue_P1 clE2
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines |
CBRCULi015-A (GM23375-1, GM23375 clone 1) Donor's gene variants: DMPK – DM1 protein kinase, DMPK – DM1 protein kinase Donor diseases: Congenital Myotonic Dystrophy CENSOi008-A (FB77R2c3, CENSOi260) Donor's gene variants: DMPK, DMPK Donor diseases: myotonic dystrophy type 1 |
| Last update | 15th December 2025 |
| User feedback | |
Provider |
|
| Generator | Radboudumc - Department of Human Genetics (RMCGEN) |
External Databases |
|
| BioSamples | SAMEA119974286 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
No |
| Subclone of | |
Donor Information
General Donor Information |
|
| Sex | male |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
External Databases (Donor) |
|
| BioSamples | SAMEA119974255 |
Ethics
Also have a look at the ethics information for the parental line
SCTCi043-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
SCTCi043-A.
|
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Reprogramming method |
|
| Vector type | Integrating |
| Vector | Virus (Lentivirus) |
| Genes | |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
No |
| Reprogramming vectors silenced? |
Yes |
| Methods used |
RT-PCR
|
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 20 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™ Flex
|
Characterisation
Analysis of Undifferentiated Cells
Differentiation Potency
In vitro directed differentiation
| Marker | Expressed |
| FOXA2 |
Yes |
| SOX17 |
Yes |
In vitro directed differentiation
| Marker | Expressed |
| HAND1 |
Yes |
| TBXT (BRACHYURY) |
Yes |
In vitro directed differentiation
| Marker | Expressed |
| PAX6 |
Yes |
| NCAM1 |
Yes |
Microbiology / Virus Screening |
|
| Mycoplasma | Negative |
Genotyping
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
46,XY
Passage number: 12
Karyotyping method:
CNV-WES
|
Other Genotyping (Cell Line) |
|
Genetic Modification
| Disease/phenotype related modifications |
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