HAD 6

The cell line is not validated yet.

General#

Cell Line

hPSCreg Name
HADe006-A
Alternative name(s)
HAD 6
Cell line type Human embryonic stem cell (hESC)
Last update 9th January 2020
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Provider

Generator Hadassah University Hospital (HAD)
Owner Hadassah Human Embryonic Stem Cell Research Center
Distributors
Derivation country Israel

External Databases

Cellosaurus CVCL_C077
BioSamples SAMEA6621258
CLO CLO_0102577
Wikidata Q54872335

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research: allowed
Clinical: not allowed
Commercial: not allowed

Donor Information#

General Donor Information

Sex unknown

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
myotonic dystrophy type 1
Myotonic Dystrophy 1
Synonyms
  • Dystrophia myotonica
  • congenital myotonic dystrophy
  • Steinert disease
  • myotonic dystrophy of Steinert
show more synonyms

External Databases (Donor)

BioSamples SAMEA6621258

Ethics#

Was the embryo established purely for research purposes? No
Have both parents consented to the use of the embryo for ESC derivation? Yes
Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? No
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Hadassah IRB Committee
Approval number 16-23.04.04
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? No

hESC Derivation#

Date of derivation 2007-03-24
Supernumerary embryos from IVF treatment?
No
Other hESC source: IVF
PGD Embryo?
Yes
Derived under xeno-free conditions?
No
Derivation under GMP?
No
Available as clinical grade?
No

Culture Conditions#

O2 Concentration 4 %
CO2 Concentration 5 %
Medium Other medium:
Base medium:
Main protein source:
Serum concentration: %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Unknown

Other Genotyping (Cell Line)