General

Cell Line
hPSCreg name	RGIe061-A
Cite as: When citing this cell line, please use the hPSCreg name (see Naming Tool ) and the corresponding Research Resource ID (RRID).	RGIe061-A (RRID:CVCL_B577)
Alternative name(s)	SI-153
Cell line type	Human embryonic stem cell (hESC)
Similar lines	SCTCi044-A-1 (iPS24-00086 clB5, fiPS_DM1Rescue_P2 clB5) Donor diseases: myotonic dystrophy type 1 SCTCi045-A-1 (iPS24-00085 clE3, fIPS_DM1Rescue_P3 clE3) Donor diseases: myotonic dystrophy type 1 SCTCi043-A-1 (iPS23-00086 clE2, fIPS_DM1Rescue_P1 clE2) Donor diseases: myotonic dystrophy type 1 SCVIi137-A (SCVI 770) Donor diseases: myotonic dystrophy type 1 CENSOi008-A (FB77R2c3, CENSOi260) Donor's gene variants: DMPK, DMPK Donor diseases: myotonic dystrophy type 1 SCTCi044-A (fIPS_DM1_P2 cl1, iPS19-00073 cl1) Donor diseases: myotonic dystrophy type 1 SCTCi045-A (iPS21-00148 cl1, fIPS_DM1_P3 cl1) Donor diseases: myotonic dystrophy type 1 CBRCULi012-A (GM04601 clone 21, GM04601-21) Donor diseases: myotonic dystrophy type 1 CBRCULi013-A (GM04602-2, GM04602 clone 2) Donor diseases: myotonic dystrophy type 1 SCVIi134-A (SCVI 692) Donor diseases: myotonic dystrophy type 1 CBRCULi003-A (59-0944-2) Donor diseases: myotonic dystrophy type 1 CBRCULi018-A (91-2032-7) Donor diseases: myotonic dystrophy type 1 CBRCULi019-A (19-1741-2) Donor diseases: myotonic dystrophy type 1 CBRCULi020-A (79-1761-15) Donor diseases: myotonic dystrophy type 1 CBRCULi007-A (14-0730-1) Donor diseases: myotonic dystrophy type 1 VUBe003-A (VUB03_DM1) Donor diseases: myotonic dystrophy type 1 RGIe057-A (SI-148) Donor diseases: myotonic dystrophy type 1 CHUQi001-A (522-2666-2, SAMEA104271492) Donor diseases: myotonic dystrophy type 1 SCTCi043-A (fIPS_DM1_P1 cl1, iPS19-00072 cl1) Donor diseases: myotonic dystrophy type 1 HADe001-A (HAD 1) Donor diseases: myotonic dystrophy type 1 CBRCULi002-A (19-1035-10) Donor diseases: myotonic dystrophy type 1 HADe006-A (HAD 6) Donor diseases: myotonic dystrophy type 1 CBRCULi004-A (79-1660-2) Donor diseases: myotonic dystrophy type 1 CBRCULi005-A (91-3158-1) Donor diseases: myotonic dystrophy type 1 CBRCULi015-A (GM23375-1, GM23375 clone 1) Donor's gene variants: DMPK – DM1 protein kinase, DMPK – DM1 protein kinase Donor diseases: Congenital Myotonic Dystrophy INNDSUi006-A Donor diseases: myotonic dystrophy type 1 ZZUi015-A (ZZU-iPS-DM1-002) Donor diseases: myotonic dystrophy type 1 KCLe014-A (KCL018_MD1) Donor diseases: myotonic dystrophy type 1
Last update	30th August 2022
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Provider
Generator	Reproductive Genetics Institute (RGI) Contact: Stem Cell Research Unit
Derivation country	United States
External Databases
Cellosaurus	CVCL_B577
Wikidata	Q54949901
General Information
Publications	Verlinsky Y et al. Human embryonic stem cell lines with genetic disorders. Reproductive biomedicine online. 2005 Jan;10(1):105-10.
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information
Sex	female
Phenotype and Disease related information (Donor)
Diseases	A disease was diagnosed. Myotonic dystrophy type 1 The donor is affected. Synonyms Dystrophia myotonica Steinert disease congenital myotonic dystrophy myotonic dystrophy of Steinert show more synonyms show less synonyms

Ethics

Alternatives to consent are available?	Yes
Alternatives to consent
Alternative consent approval number
How may genetic information associated with the cell line be accessed?

hESC Derivation

Supernumerary embryos from IVF treatment?	Yes
PGD Embryo?	Yes

Culture Conditions

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	Flow Cytometry	Enzymatic Assay	Expression Profiles
Alkaline Phosphatase	Yes
POU5F1 (OCT-4)	Yes
SSEA-3	Yes
SSEA-4	Yes
TRA 1-60	Yes
TRA 1-81	Yes
TRA 2-39	Yes

Genotyping

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?	Yes 46XX Passage number: 7
Other Genotyping (Cell Line)