KCL018_MD1
KCLe014-A
General
Cell Line |
|
| hPSCreg name | KCLe014-A |
| Cite as: | KCLe014-A (RRID:CVCL_A251) |
| Alternative name(s) |
KCL018_MD1
|
| Cell line type | Human embryonic stem cell (hESC) |
| Similar lines |
CBRCULi015-A (GM23375-1, GM23375 clone 1) Donor's gene variants: DMPK – DM1 protein kinase, DMPK – DM1 protein kinase Donor diseases: Congenital Myotonic Dystrophy CENSOi008-A (FB77R2c3, CENSOi260) Donor's gene variants: DMPK, DMPK Donor diseases: myotonic dystrophy type 1 |
| Last update | 28th July 2017 |
| User feedback | |
Provider |
|
| Generator |
King's College London (KCL)
Contact:
Department of Women's Health |
| Derivation country | United Kingdom |
External Databases |
|
| Cellosaurus | CVCL_A251 |
| NIHhESC | NIHhESC-13-0218 |
| Wikidata | Q54899665 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes |
Donor Information
General Donor Information |
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Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
Ethics
| Alternatives to consent are available? | Yes |
| Alternatives to consent | |
| Alternative consent approval number | NIHhESC-13-0218 |
| Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? | Yes |
| Name of accrediting authority involved? | NRES |
hESC Derivation
| Date of derivation | 2010-04-22 |
| Embryo stage | Blastula with ICM and Trophoblast |
| Supernumerary embryos from IVF treatment? |
Yes
|
| PGD Embryo? |
Yes |
| Expansion status |
6
|
| ICM morphology |
B
|
| Trophectoderm morphology |
b
|
| ZP removal technique | Mechanical |
| Cell isolation | Laser |
| Cell seeding | Isolated ICM |
Culture Conditions
| Surface coating | Cellstart | ||||||
| Feeder cells |
Human Foreskin Fibroblast Cellfinder Ont Id: CELDA_000001419 |
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| O2 Concentration | 5 % | ||||||
| CO2 Concentration | 6 % | ||||||
| Medium |
Other medium:
Base medium: KOSR-XF
Main protein source: Knock-out serum replacement Serum concentration: 15 % Supplements
|
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| TRA 1-60 |
Yes |
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| TRA 1-81 |
Yes |
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Differentiation Potency
Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
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