CENSOi008-A

FB77R2c3, CENSOi260

General#

Cell Line

hPSCreg Name CENSOi008-A
Alternative name(s)
FB77R2c3, CENSOi260
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 19th January 2018
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Provider

Generator Censo Biotechnologies (CENSO)
Distributors

External Databases

BioSamples SAMEA104133816
Cellosaurus CVCL_LE91
ECACC 66540603
EBiSC CENSOi008-A

General Information

Is the cell line available in principle? Available with restrictions: Available for non-commercial research

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
myotonic dystrophy type 1
Synonyms
  • Dystrophia myotonica
  • congenital myotonic dystrophy
  • Steinert disease
  • myotonic dystrophy of Steinert
show more synonyms

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

External Databases (Donor)

BioSamples SAMEA104133817

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Age of donor (at collection) 25-29
Collected in 2015

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA
mRNA

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
SSEA-1
No
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
MIXL1
Yes
GATA6
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
NCAM1
Yes
VIM
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
Yes
NeuroD1
Yes
HES5
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
No autosomal or sex aneuploidies were detected in this sample
Karyotyping method: KaryoLite BoBs

Other Genotyping (Cell Line)