Generation of four iPSC lines from Neurofibromatosis Type 1 patients
We describe the generation and characterisation of four human induced pluripotent stem cell (iPSC) lines from peripheral blood mononuclear cells (PBMC) from individuals with neurofibromatosis type (NF1). PBMC reprogramming was performed using a non-integrative Sendai virus containing the reprogramming factors OCT4, SOX2, MYC and KLF4. All iPSC lines exhibited a normal karyotype, and pluripotency was validated by flow cytometry (EPCAM, TRA-1-81, SSEA1 and CD9) and immunofluorescence (OCT4 and Nanog). Differentiation of the cells into the three embryonic germ layers was confirmed using immunofluorescence. These iPSC lines are a valuable pre-clinical resource to study the molecular mechanisms underlying NF1. Copyright © 2020. Published by Elsevier B.V.
|Authors||Bozaoglu K, Shern Lee W, Haebich KM, North KN, Payne JM, Lockhart PJ|
|Journal||Stem cell research|
|Publication Date||2020 Dec;49:102013|