Derivation of a human induced pluripotent stem cell (hiPSC) line (PUMCSMi001-A) from peripheral blood mononuclear cells of one healthy female donor


Human induced pluripotent stem cells (hiPSCs) are valuable models for studying the molecular pathogenesis of inherited diseases and key tools for cell therapy. We derived peripheral blood mononuclear cells (PBMCs) isolated from a 30-year-old healthy female donor using integration-free Sendai virus to generate a hiPSC line, PUMCSMi001-A, which can serve as a control for the studies of pathogenesis of cardiovascular disease and drug screening. Quality assessment confirmed that the cell line PUMCSMi001-A expressed pluripotency genes, had normal karyotype and differentiation potential into three layers in vivo. Copyright © 2021. Published by Elsevier B.V.

Authors Du M, Li L, Hu Y, Mi H, Wang T
Journal Stem cell research
Publication Date 2021 May;53:102312
PubMed 33812336
DOI 10.1016/j.scr.2021.102312

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