NP0040-8

General#

Cell Line

hPSCreg Name UKKi011-A
Alternative name(s)
NP0040-8
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 8th December 2017
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Provider

Generator Klinikum der Universität zu Köln (UKK)
Owner Institute for Neurophysiology, Medical Faculty
Distributors

External Databases

BioSamples SAMEA2774610
Cellosaurus CVCL_9S63
ECACC 66540010
EBiSC UKKi011-A

General Information

Projects
Is the cell line available in principle? Available, without restrictions

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes
  • Healthy donor

External Databases (Donor)

BioSamples SAMEA2774627

Ethics#

We are currently revising this section.

hIPSC Derivation#

General

Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • skin zone
  • region of skin
  • skin region
  • skin
show more synonyms
Collected in 2013
Passage number reprogrammed P4

Reprogramming method

Vector type Non-integrating
Vector Episomal
Genes
Vector map

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones human ESC-like morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 5 %
Medium Essential 8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Unkown
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Unkown
SSEA-4
Yes
TRA 1-60
Unkown
ZFP42 (REX-1)
Unkown
TRA 1-80
Yes
Morphology pictures
brightfield image of NP0040-8 iPSC colonies; 10x objective
brightfield image of NP0040-8 iPSC colony at higher magnification; 32x objective
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX17
Yes
FOXA2
No
GSC
No
GATA6
Yes
CXCR4
Yes
PITX1
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
HAND1
Yes
VIMENTIN
Yes
BMP4
Yes
DCN
Yes
PECAM
Yes
PDGF
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
PAX6
No
Sox1
No
B-TUBULIN
No
NEUROD1
Yes
HES5
Yes
PDGF
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Not done
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Karyotyping method: Molecular karyotyping using OmniExpress Exome Chip

Other Genotyping (Cell Line)