HHUi006-B

General

Cell Line
hPSCreg Name	HHUi006-B
Alternative name(s)	11656_K
Cell line type	Human induced pluripotent stem cell (hiPSC)
Last update	24th August 2022
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Provider
Generator	Universitätsklinikum Düsseldorf (HHU)
Owner	Universitätsklinikum Düsseldorf (HHU)
Derivation country	Germany
External Databases
BioSamples	SAMEA110696888
CLO	CLO_0103965
Cellosaurus	CVCL_C1TC
Wikidata	Q114311618
General Information
Publications	Steiner T et al. RNA-based generation of iPSCs from a boy carrying the mutation m.9185 T>C in the mitochondrial gene MT-ATP6 and from his healthy mother. Stem cell research. 2022 Oct;64:102920.
* Is the cell line readily obtainable for third parties?	No

Donor Information

General Donor Information

Sex

female

Age of donor (at collection)

40-44

Ethnicity

Caucasian

Phenotype and Disease related information (Donor)

Diseases

No disease was diagnosed.

Family history

Carrier of mitochondrial DNA mutation MT-ATP6 (m.9185T>C) in the MT-ATP6 gene at low heteroplasmy levels. Has son with maternally inherited Leigh syndrome (MILS) due to homoplasmic MT-ATP6 mutation (m.9185T>C).

Karyotyping (Donor)

Has the donor karyotype been analysed?

Yes

46,XX

Fibs_11656_HHUi006.pdf

Karyotyping method: BeadArray technology (Illumina)

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?

No

Donor Relations

Other cell lines of this donor

HHUi006-A

All cell lines of this donor's relatives

Has son:

External Databases (Donor)

BioSamples

SAMEA110696884

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived?	Yes
Was the consent voluntarily given?	Yes
Has the donor been informed that participation will not directly influence their personal treatment?	Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor?	Yes
Do you (Depositor/Provider) hold the original Donor Consent Form?	Yes
Confirm that consent was obtained by a qualified professional	Yes
Has the donor been informed about how her/his data will be protected?	Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised.	anonymised
Does consent explicitly allow the derivation of pluripotent stem cells?	Yes
Does consent expressly prevent the derivation of pluripotent stem cells?	No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world?	Yes
How may genetic information associated with the cell line be accessed?	Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample?	No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions?	Yes
Name of accrediting authority involved?	Ethic Committee of the Medical Faculty of Heinrich Heine University, Düsseldorf
Approval number	Study number: 2020-967_2
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells?	Yes
Name of accrediting authority involved?	Ethic Committee of the Medical Faculty of Heinrich Heine University, Düsseldorf
Approval number	Study number: 2020-967_2
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General
Source cell type	Fibroblast Fibroblast in the OLS Synonyms fibroblast Fibroblasts Fibroblast FIBROBLAST show more synonyms show less synonyms
Age of donor (at collection)	40-44
Passage number reprogrammed	6
Reprogramming method
Vector type	Non-integrating
Vector	Self-replicating RNA vector
Genes	POU5F1 SOX2 KLF4 MYC GLIS1
Vector free reprogramming
Other
Derived under xeno-free conditions	Unknown
Derived under GMP?	Unknown
Available as clinical grade?	Unknown

Culture Conditions

Surface coating	Matrigel/Geltrex
Feeder cells	No
Passage method	Mechanically
O2 Concentration	5 %
CO2 Concentration	5 %
Medium	mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?	Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?	No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?	Yes

Characterisation

Analysis of Undifferentiated Cells

Marker Expression

Marker	Expressed	Immunostaining	RT-PCR	FACS	Enzymatic Assay	Expression Profiles
NANOG	Yes
TRA 1-60	Yes
SOX2	Yes
POU5F1 (OCT-4)	Yes
DNMT3B	Yes

Morphology

Morphology pictures

ips_4x11656_K_p3_scale400um.pdf

iPSC 11656_K, passage 3, acquired with brightfield microscope Nikon eclipse TS2, scale bar 400Âµm

Other pluripotency tests used

Embryoid bodies (EBs) formation and differentiation: Assay confirmed that the iPSC line was able to differentiate into cell types of the three germ layers, as shown by markers for mesoderm (smooth muscle actin, SMA and fibronectin, FN1) ectoderm (PAX6 and TUJ1), and endoderm (alpha-fetoprotein, AFP and SOX17).