A4_C1

General

Cell Line

hPSCreg Name
HHUi003-B
Alternative name(s)
A4_C1
Cell line type Human induced pluripotent stem cell (hiPSC)
Last update 24th August 2022
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Provider

Generator Universitätsklinikum Düsseldorf (HHU)
Owner Universitätsklinikum Düsseldorf (HHU)
Derivation country Germany

External Databases

BioSamples SAMEA110696885
CLO CLO_0103962
Cellosaurus CVCL_C1T9

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex male
Age of donor (at collection) 1-4
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
maternally-inherited Leigh syndrome
Mitochondrial DNA mutation of MT-ATP6: m.9185T>C
The donor is a carrier of a disease-associated mutation and affected.
Family history The healthy mother carries the same mitochondrial DNA mutation of the gene MT-ATP6 (m.9185T>C) with low heteroplasmy levels.

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
46,XY
Karyotyping method: BeadArray technology (Illumina)

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

Donor Relations

Other cell lines of this donor
All cell lines of this donor's relatives

External Databases (Donor)

BioSamples SAMEA110696883

Ethics

Has informed consent been obtained from the donor of the embryo/tissue from which the pluripotent stem cells have been derived? Yes
Was the consent voluntarily given? Yes
Has the donor been informed that participation will not directly influence their personal treatment? Yes
Can you provide us with a copy of the Donor Information Sheet provided to the donor? Yes
Do you (Depositor/Provider) hold the original Donor Consent Form? Yes
Confirm that consent was obtained by a qualified professional Yes
Has the donor been informed about how her/his data will be protected? Yes
Please indicate whether the data associated with the donated material has been pseudonymised or anonymised. anonymised
Does consent explicitly allow the derivation of pluripotent stem cells? Yes
Does consent expressly prevent the derivation of pluripotent stem cells? No
Does consent prevent the DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
Does consent prevent CELLS DERIVED FROM THE DONATED BIOSAMPLE from being made available to researchers anywhere in the world? Yes
How may genetic information associated with the cell line be accessed? Controlled Access
Will the donor expect to receive financial benefit, beyond reasonable expenses, in return for donating the biosample? No
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the Research Protocol including the consent provisions? Yes
Name of accrediting authority involved? Ethic Committee of the Medical Faculty of Heinrich Heine University, Düsseldorf
Approval number Study number: 2020-967_2
Has a favourable opinion been obtained from a research ethics committee, or other ethics review panel, in relation to the PROPOSED PROJECT, involving use of donated embryo/tissue or derived cells? Yes
Name of accrediting authority involved? Ethic Committee of the Medical Faculty of Heinrich Heine University, Düsseldorf
Approval number Study number: 2020-967_2
For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used?

hIPSC Derivation

General

Source cell type
Fibroblast
Synonyms
  • fibroblast
  • Fibroblasts
  • Fibroblast
  • FIBROBLAST
show more synonyms
Age of donor (at collection) 1-4
Passage number reprogrammed 5

Reprogramming method

Vector type Non-integrating
Vector Self-replicating RNA vector 
Genes

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions

Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
O2 Concentration 5 %
CO2 Concentration 5 %
Medium mTeSR™ 1
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
DNMT3B
Yes
Score:
Marker Present Absent
mCpG
OCT4
Morphology pictures
ipsc_4x.A4_C1_p3_scale400um.pdf
iPSC A4_C1, passage 3, acquired with brightfield microscope Nikon eclipse TS2, scale bar 400µm
Embryoid bodies (EBs) formation and differentiation: Assay confirmed that the iPSC line was able to differentiate into cell types of the three germ layers, as shown by markers for mesoderm (smooth muscle actin, SMA and fibronectin, FN1) ectoderm (PAX6 and TUJ1), and endoderm (alpha-fetoprotein, AFP and SOX17).
Differentiation Potency
SOX17
Ont Id: OGG_3000064321
In vitro spontaneous differentiation
Alpha-Fetoprotein
Ont Id: NCIT_C16278
In vitro spontaneous differentiation
Fibronectin
Ont Id: NCIT_C16581
In vitro spontaneous differentiation
ACTA2
Ont Id: OGG_3000000059
In vitro spontaneous differentiation
PAX6
Ont Id: OGG_3000005080
In vitro spontaneous differentiation
Beta Tubulin
Ont Id: NCIT_C129655
In vitro spontaneous differentiation

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Certificate of Analysis

Is there a certificate of analysis available?
Yes
Passage:

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XY
Passage number: 5
Karyotyping method: BeadArray technology (Illumina)

Other Genotyping (Cell Line)