UGENTi002-A-1
General
Cell Line |
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| hPSCreg name | UGENTi002-A-1 |
| Cite as: | UGENTi002-A-1 (RRID:CVCL_D0MN) |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
| Last update | 3rd July 2024 |
| User feedback | |
Provider |
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| Generator |
Ghent University (UGENT)
Contact:
Ghent University (UGENT) |
| Owner | Ghent University (UGENT) |
| Distributors | |
| Derivation country | Belgium |
External Databases |
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| BioSamples | SAMEA113958745 |
| Cellosaurus | CVCL_D0MN |
| Wikidata | Q123033697 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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| Subclone of | |
Donor Information
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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External Databases (Donor) |
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| BioSamples | SAMEA113939457 |
Ethics
Also have a look at the ethics information for the parental line
UGENTi002-A
.
| For generation of the cell line, who was the supplier of any recombined DNA vectors or commercial kits used? |
hIPSC Derivation
General |
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The source cell information can be found in the parental cell line
UGENTi002-A.
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Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
| Is reprogramming vector detectable? |
Yes |
| Methods used |
RT-PCR
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Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Selection criteria for clones | Flat morphology with rounded edges, desirable growth, expression of pluripotency markers |
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions
| Surface coating | Matrigel/Geltrex | ||||||
| Feeder cells |
No |
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| Passage method |
Enzymatically
TrypLE
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| O2 Concentration | 5 % | ||||||
| CO2 Concentration | 5 % | ||||||
| Medium |
Essential 8™
Supplements
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
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| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
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| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| SOX2 |
Yes |
Score:
| Marker | Present | Absent |
| mCpG | ||
| OCT4 |
Morphology pictures
morphology.tif
scalebar = 400µm
Differentiation Potency
In vitro directed differentiation
In vitro directed differentiation
Genotyping
Karyotyping (Cell Line) |
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Other Genotyping (Cell Line) |
Genetic Modification
| Disease/phenotype related modifications |
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