Insertion of a FLAG-tag sequence at the end of exon 9 of the TBX5 gene in three induced pluripotent stem cell lines (DHMi004-A-4, DHMi004-A-5, DHMi004-A-6) by CRISPR/Cas9 technology


The identification of TBX5-related regulatory sequences in genes essential for heart development is hampered by the absence of antibodies which allow precipitation of TBX5:DNA complexes. Employing CRISPR/Cas9 technology, we have inserted a FLAG-tag sequence at the end of exon 9 of the TBX5 gene prior to the stop codon by homologous recombination. The translated TBX5-FLAG fusion protein of the three iPSC lines can effectively be precipitated by anti-FLAG antibodies and, thus, allow the detection of specific TBX5-binding sites and their associated genes. Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.

Authors Lahm H, Singh NK, Gottmann I, Doppler SA, Dzilic E, Preisler H, Schneider S, Lange R, Krane M, Dreßen M
Journal Stem cell research
Publication Date 2024 Feb;74:103261
PubMed 38100916
DOI 10.1016/j.scr.2023.103261

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