GENEAe004-A

SIVF05, GENEA005

The cell line is not validated yet.

General#

Cell Line

hPSCreg Name GENEAe004-A
Alternative name(s)
SIVF05, GENEA005
Cell line type Human embryonic stem cell (hESC)
Last update 10th April 2017
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Provider

Generator Genea (GENEA)
Contact:
Biocells
Derivation country Australia

External Databases

BioSamples SAMEA104012406
Cellosaurus CVCL_9014

General Information

Publications
Is the cell line available in principle?
No

Donor Information#

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

External Databases (Donor)

BioSamples SAMEA104012405

Ethics#

We are currently revising this section.

hESC Derivation#

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes
ZP removal technique Enzymatic
Cell isolation Mechanical
Cell seeding Isolated ICM

Culture Conditions#

Passage method Mechanically
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Fetal bovine serum
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX

Other Genotyping (Cell Line)