GENEAe004-A

SIVF05, GENEA005

The cell line is not validated yet.

General

Cell Line

hPSCreg name GENEAe004-A
Cite as:
GENEAe004-A (RRID:CVCL_9014)
Alternative name(s)
SIVF05, GENEA005
Cell line type Human embryonic stem cell (hESC)
Similar lines No similar lines found.
Last update 22nd May 2019
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Provider

Generator Genea (GENEA)
Contact:
Biocells
Derivation country Australia

External Databases

BioSamples SAMEA104012406
Cellosaurus CVCL_9014
Wikidata Q54835567

General Information

Publications
* Is the cell line readily obtainable for third parties?
No

Donor Information

General Donor Information

Sex female

Phenotype and Disease related information (Donor)

Diseases

External Databases (Donor)

BioSamples SAMEA104012405

Ethics

Alternatives to consent are available? Yes
Alternatives to consent
Name of accrediting authority involved?
Approval number

hESC Derivation

Embryo stage Blastula with ICM and Trophoblast
Supernumerary embryos from IVF treatment?
Yes
PGD Embryo?
Yes
ZP removal technique Enzymatic
Cell isolation Mechanical
Cell seeding Isolated ICM

Culture Conditions

Passage method Mechanically
Medium Other medium:
Base medium: DMEM/F-12
Main protein source: Fetal bovine serum
Serum concentration: 20 %
Supplements
L-Glutamine
bFGF

Characterisation

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
POU5F1 (OCT-4)
Yes
SSEA-4
Yes
TRA 1-60
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation

Genotyping

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46XX
Passage number: 28

Other Genotyping (Cell Line)