Generation of a homozygous CRISPR/Cas9-mediated knockout human iPSC line for the STUB1 locus
STUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. For ideal in vitro disease modelling with isogenic controls, we generated a CHIP knockout cell line from a healthy control with no CHIP functionality, but remaining genomic integrity and verified pluripotency. Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.
|Authors||Schuster S, Saravanakumar S, Schöls L, Hauser S|
|Journal||Stem cell research|
|Publication Date||2019 Jan;34:101378|